Leptospira: a spirochaete with a hybrid outer membrane
نویسندگان
چکیده
منابع مشابه
amplification and cloning of a gene encoding a 41 kda outer membrane protein (lipl41) of leptospira interrogans serovar canicola
background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...
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The outer membranes of invasive spirochetes contain unusually small amounts of transmembrane proteins. Pathogenic Leptospira species produce a rare 31-kDa surface protein, OmpL1, which has a deduced amino acid sequence predictive of multiple transmembrane beta-strands. Studies were conducted to characterize the structure and function of this protein. Alkali, high-salt, and urea fractionation of...
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Leptospirosis is a zoonosis with worldwide distribution caused by pathogenic spirochetes belonging to the genus Leptospira. The leptospiral life cycle involves transmission via fresh water and colonization of the renal tubules of their reservoir hosts or infection of accidental hosts, including humans. Bacterial outer membrane proteins (OMPs), particularly those with surface-exposed regions, pl...
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A cecropin/melittin hybrid peptide (CEME) produced by recombinant DNA procedures was tested for its ability to interact with the outer membrane of Pseudomonas aeruginosa and found to have identical biological properties to that of chemically synthesized CEME. CEME was shown to kill P. aeruginosa and permeabilize its outer membrane to lysozyme and 1-N-phenylnaphthlyamine, in some cases better th...
متن کاملExpression and characterization of recombinant leptospiral outer membrane protein LipL32 from Leptospira interrogans serovar autumnalis.
Leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in Thailand, was isolated from a patient for DNA extraction and amplification of LipL32 gene by polymerase chain reaction (PCR). The 782 bp PCR product was obtained, which was inserted into pAE plasmid with polyhistidine (His6 tag) to construct pAE-LipL32. This recombinant plasmid was transfected into E. coli BL21 (DE...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2010
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.2010.07262.x